nrdR [2019-07-09 08:23:54]

negative regulator of transcription of the ribonucleotide reductase nrd operon

locus: BSU29000

pI: 8.05

mw: 17.77 kDa

protein length: 152 aa

gene length: 459 bp

function: control of ribonucleotide reductase expression

product: transcription repressor of the ribonucleotide reductase nrd operon

essential: no

synonyms: ytcG

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.5|Transcription factors/ other]

Gene

Coordinates: 2,965,681 → 2,966,139

The protein

Protein family

• nrdR family (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|FD4E988BB73F6476CCEA4C521F343D6F8CBA457F|nrdR]
  description: [Pubmed|9387221]

  regulation

  • constitutively expressed [Pubmed|27920297]

  additional information

  • the terminator is [protein|search|NusA]-dependent [ http://www.nature.com/articles/nmicrobiol20157 Reference]

Biological materials

Mutant

• MGNA-A123 (ytcG::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/123 NBRP B. subtilis, Japan]
• BKE29000 (Δ[gene|FD4E988BB73F6476CCEA4C521F343D6F8CBA457F|nrdR]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE29000 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATAAAATCAGCTCCCAAC, downstream forward: _UP4_TAAAAGAGCTAAGAGGATTC
• BKK29000 (Δ[gene|FD4E988BB73F6476CCEA4C521F343D6F8CBA457F|nrdR]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK29000 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATAAAATCAGCTCCCAAC, downstream forward: _UP4_TAAAAGAGCTAAGAGGATTC

References

• 16950922,9387221,15949864,27920297,28624879